521-31-3

  • Product NameLuminol
  • Molecular FormulaC8H7N3O2
  • Molecular Weight177.162
  • Purity99%
  • Appearanceyellow crystals or beige powder
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Product Details

Quick Details

  • CasNo: 521-31-3
  • Molecular Formula: C8H7N3O2
  • Appearance: yellow crystals or beige powder
  • Purity: 99%

Reputable manufacturer supply Luminol 521-31-3 in stock with high standard

  • Molecular Formula:C8H7N3O2
  • Molecular Weight:177.162
  • Appearance/Colour:yellow crystals or beige powder 
  • Melting Point:319-320 °C 
  • Refractive Index:1.6500 (estimate) 
  • Boiling Point:621.9oC at 760 mmHg 
  • PKA:(Calcd.) 10.50 ± 0.20;;0.58 ± 0.20(at 25℃) 
  • Flash Point:329.9oC 
  • PSA:91.74000 
  • Density:1.433 g/cm3 
  • LogP:0.37980 

3-Aminophthalhydrazide(Cas 521-31-3) Usage

Chemical fluorescent molecules

Luminol?is an organic compound which, when oxidized, emits light — a phenomenon known as?chemiluminescence.??This is similar to the reactions that fireflies uses to emit light, and to those used in "glow-sticks" and some roadside emergency lights.?In this reaction, a small amount of luminol?(3-aminophthalhydrazide or 5-amino-2,3-dihydro- 1,4-phthalazinedione) is dissolved in a basic aqueous solution, which also contains a small amount of copper(II) sulfate.? To this solution is added a solution of a mild oxidizing agent, which is 0.3% hydrogen peroxide in the demonstration below.? (Bleach is also used in some recipes as the oxidizing agent.)??The reaction is believed to occur by the following mechanism:

Chemiluminescence method

Luminol chemiluminescence has been trialed for estimating the PMI from skeletal remains. Luminol methods are based on the reaction between luminol and hydrogen peroxide which is catalyzed by iron in hemoglobin found in fluids and tissues from bodies. This reaction results in a chemiluminescence light which can be measured by various means .

Chemical properties

Yellow crystalline powder, Easily soluble in lye, soluble in dilute acid but almost insoluble in water and hardly soluble in alcohol. When its neutral or weak acidic solution is exposed to ultraviolet light, it exhibits strongly bright blue fluorescence. Melting point: 329-332 ° C

Hazards & Safety Information

Category Toxic substances Toxic grading poisoning Acute toxicity Oral – rat; LD50: > 500 mg/kg Flammability and Hazardous properties it is combustible with combustion producing toxic nitrogen oxide fumes Storage and transportation characteristics warehouse: ventilated, low temperature and dry Fire extinguishing agent dry powder, foam, sand, carbon dioxide, mist water

General Description

Yellow crystals or light beige powder.

Air & Water Reactions

Insoluble in water.

Reactivity Profile

Oxidation of 3-Aminophthalhydrazide is accompanied by a striking emission of light. . .

Health Hazard

ACUTE/CHRONIC HAZARDS: When heated to decomposition 3-Aminophthalhydrazide emits toxic fumes.

Fire Hazard

Flash point data for 3-Aminophthalhydrazide are not available, but 3-Aminophthalhydrazide is probably combustible.

Synthesis

The synthesis of Luminol is as follows: To a reaction tube, add 140 mg of 3-nitrophthalhydrazide and 1.0 mL of 3 M sodium hydroxide solution. Stir with a rod, and to the resulting deep brown-red solution add 0.6 g of sodium hydrosulfite dihydrate (Na2S2O4 · 2 H2O, MW 210.2). Wash down the sides of the tube with a small amount of water. Heat to a gentle boil and keep the tube hot for 5 minutes. During this time some product may begin to crystallize. Add 0.4 mL of acetic acid, cool the tube in cold water, and stir. Collect the light-yellow product?Luminol by suction filtration.

Purification Methods

Dissolve luminol in KOH solution, treat with Norit (charcoal), filter and precipitate it with conc HCl. [Hardy et al. Talanta 24 297 1977.] Store it in the dark in an inert atmosphere, because its structure changes during its luminescence. It has been recrystallised from 0.1M KOH [Merenyi et al. J Am Chem Soc 108 77716 1986]. [Beilstein 25 II 389, 25 III/IV 4192.]

InChI:InChI=1/C8H7N3O2/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12/h1-3H,9H2,(H,10,12)(H,11,13)

521-31-3 Relevant articles

Physico-chemical properties of isomeric forms of luminol in aqueous solutions

Skripnikova, Tatiana A.,Lysova, Svetlana S.,Zevatskii, Yuriy E.,Myznikov, Leonid V.,Vorona, Svetlana V.,Artamonova, Tatiana V.

, p. 59 - 63 (2018)

Two luminol isomers, 2,4-diaminoisoindol...

A Common, Facile and Eco-Friendly Method for the Reduction of Nitroarenes, Selective Reduction of Poly-Nitroarenes and Deoxygenation of N-Oxide Containing Heteroarenes Using Elemental Sulfur

Cerecetto, Hugo,Romero, Angel H.

, (2020/03/23)

A transition metal-free, environment-fri...

Luminol or isoluminol synthesizing method by one-pot process

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Paragraph 0062, (2017/08/30)

The invention discloses a luminol or iso...

CRYSTALLINE FORM OF 5-AMINO-2,3-DIHYDROPHTHALAZINE-1,4-DIONE

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Page/Page column 23; 24, (2017/09/08)

A new crystalline form of 5-amino-2,3-di...

521-31-3 Process route

5-nitro-2,3-dihydro-phthalazine-1,4-dione
3682-15-3

5-nitro-2,3-dihydro-phthalazine-1,4-dione

5-amino-2,3-dihydrophthalazine-1,4-dione
521-31-3

5-amino-2,3-dihydrophthalazine-1,4-dione

Conditions
Conditions Yield
With sulfur; sodium hydroxide; In methanol; water; at 80 ℃; for 1h; Green chemistry;
89%
With formic acid; palladium 10% on activated carbon; hydrogen; at 35 ℃; for 5h; Reagent/catalyst;
83%
With iron; ammonium chloride; In water;
 
5-nitro-2,3-dihydro-phthalazine-1,4-dione; With sodium hydroxide; In water; at 50 - 60 ℃;
With sodium dithionite; In water; at 50 ℃; for 4h; Reflux;
With acetic acid; In water;
 
monosodium 5-amino-1,4-dioxo-3,4-dihydro-1H-phthalazin-2-ide

monosodium 5-amino-1,4-dioxo-3,4-dihydro-1H-phthalazin-2-ide

5-amino-2,3-dihydrophthalazine-1,4-dione
521-31-3

5-amino-2,3-dihydrophthalazine-1,4-dione

Conditions
Conditions Yield
With hydrogenchloride; In water;
 

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